Journal: Development (Cambridge, England)

Article Title: Requirements for endoderm and BMP signaling in sensory neurogenesis in zebrafish.

doi: 10.1242/dev.01936

Figure Lengend Snippet: Fig. 5. BMP gene expression in the pharyngeal pouches during epibranchial development, and defects in cas mutants. Lateral views, anterior to the left. Whole- mount in situ hybridization was performed in wild types at 22 hpf (top row), 30 hpf (second row) and 40 hpf (third row), and in cas mutants at 40 hpf (bottom row), for bmp2a (A,E,I,M), bmp2b (B,F,J,N), bmp4 (C,G,K,O) and bmp5 (D,H,L,P). Arrows indicate initial expression at the ventrolateral edges of each pouch. Arrow in H denotes bmp5 expression in the dorsal pouches. 1-5, pharyngeal pouches; ot, otic capsule.

Article Snippet: Human recombinant BMP4, BMP5, BMP7 and Noggin proteins (R&D Systems) were used for bead implantation experiments.

Techniques: Gene Expression, In Situ Hybridization, Expressing

Journal: Development (Cambridge, England)

Article Title: Requirements for endoderm and BMP signaling in sensory neurogenesis in zebrafish.

doi: 10.1242/dev.01936

Figure Lengend Snippet: Fig. 8. BMP4-coated beads induce, and NOG-coated beads inhibit, epibranchial formation. Lateral views, anterior to the left. In situ hybridization for phox2b mRNA to label epibranchials at 48 hpf. (A) Wild-type control embryo. (B-F) Embryos that received Affi-Gel Blue beads soaked in either PBS (B), BMP4 (10 μg/ml, C; 1 μg/ml, D) or NOG protein (500 pg/ml, E; 300 pg/ml, F). Arrows in C and D indicate ectopic phox2b+ cells adjacent to the bead; arrows in E and F denote missing ganglia adjacent to the NOG-coated bead. Asterisks indicate beads. gVII, facial; gIX, glossopharyngeal; gX, vagal; ot, otic vesicle. Scale bar: 50 μm.

Article Snippet: Human recombinant BMP4, BMP5, BMP7 and Noggin proteins (R&D Systems) were used for bead implantation experiments.

Techniques: In Situ Hybridization, Control

Journal: mAbs

Article Title: Discovery of ginisortamab, a potent and novel anti-gremlin-1 antibody in clinical development for the treatment of cancer

doi: 10.1080/19420862.2023.2289681

Figure Lengend Snippet: Ginisortamab IC50 for the inhibition of gremlin-1 antagonism of BMP-4/7 heterodimer activity, as measured in the BMP signaling reporter gene assay.

Article Snippet: Human gremlin-1 (R&D Systems, 5190-GR) or mouse gremlin-1 (R&D Systems, 956-GR) protein was added to the antibody titration and pre-incubated for 45 min at 37°C, 5% CO 2 , followed by a second pre-incubation with BMP-4/-7 heterodimer (R&D Systems, 3727-BP), BMP-4 (R&D Systems, 314-BP), or BMP-7 (R&D Systems, 354-BP) for 45 min at 37°C, 5% CO 2 , prior to addition to the cells.

Techniques: Inhibition, Activity Assay